DNA

Part:BBa_K676009

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)


Gyrase B

This is the 2414bp of GyrB ORF . GyrB subunit has two important lysine residues at 103 and 110th positions which interact and coordinate the positioning of ATP such that the binding and hydrolysis of the ATP will result in the conformational changes which introduce negative supercoiling into the DNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
    Illegal BglII site found at 1667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
    Illegal AgeI site found at 931
    Illegal AgeI site found at 2047
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None